yonN [2019-05-02 18:01:36]

SP prophage-derived DNA-binding protein HU 2

locus: BSU21050

pI: 10.23

mw: 9.71 kDa

protein length: 92 aa

gene length: 279 bp

function: untwisting of the DNA double helix

product: DNA-binding protein HU 2

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.8|Genetics/ other/ based on similarity]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]

Gene

Coordinates: 2,225,337 2,225,615

The protein

Catalyzed reaction/ biological activity

• [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|YonN]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|Nth] endonuclease activity at AP sites [Pubmed|21954439]

Protein family

• glycosyltransferase 1 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|Hbs]

Structure

• [PDB|1HUE] ([protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|Hbs] from B. stearothermophilus, 71% identity) [pubmed|7500343]

Expression and Regulation

Operons

• genes: [gene|121C3C9CF49711316A526B9571F020BE8E304B31|yonP]-[gene|34380A2F469ACFD90D450717A41D6F5CC19DCD7E|yonO]-[gene|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE21050 ([gene|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAATTTCCTCCTAA, downstream forward: _UP4_TAATCAGTTAATGAGGACGA
• BKK21050 ([gene|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAATTTCCTCCTAA, downstream forward: _UP4_TAATCAGTTAATGAGGACGA

References

• 21954439,7500343